Laboratory experiment extraction of DNA from strawberries Essay Example For Students

Laboratory experiment extraction of DNA from strawberries Essay Introduction Deoxyribonucleic acid, short for deoxyribonucleic acid, is present in all life organisms’ cells. It contains a set of instructions that direct the activities of the organism’s development and map. ( Buddies, 2013 ) It is located within a karyon ; a membrane-bound cell organ. ( University of Queensland, 2011 ) Surrounding the karyon, there is the cell wall, chondriosomes, vacuoles, endoplasmic Reticulum, Golgi setup, lysosomes and etc. Deoxyribonucleic acid is composed of three parts: nitrogen-bearing bases ( A, T, G and C ) , sugars and phosphates which form a dual spiral with the linkage of H bonds. ( University of Queensland, 2011 ) In this lab experiment, fresh strawberries were used because strawberry cells could give more Deoxyribonucleic acid than any other fruit for the ground that they are octoploids. ( Buddies, 2013 ) This means that each strawberry cell has eight transcripts its genome ; hence they are able to supply a greater sum of extracted DNA for pupils to see. ( Buddies, 2013 ) Strawberries besides contain bantam enzymes, pectinases and cellulases, which are able to assist to interrupt down cell walls and do the DNA extraction procedure easier and more efficient. ( Sweeney, 2012 ) This activity will show how Deoxyribonucleic acid can be separated from all the other parts of the cell utilizing common family stuffs. The intent of this experiment is to larn how to pull out strawberry Deoxyribonucleic acid by merely utilizing mundane family merchandises and for pupils to see a big sample of seeable Deoxyribonucleic acid because it is predicted that strawberry DNA will be successfully extracted and will go seeable to the bare oculus. Materials and Methods: Three strawberries were put into the microwave and heated for approximately 12 seconds. Next, green roots were removed, and the strawberries were crushed and mashed into strawberry juice by a liquidizer alternatively of utilizing custodies for a better consequence. Then, the strawberry juice was poured into a Ziploc bag along with 2 pip-squeaks of buffer ( a mixture of Dawn dish soap, salt and tap H2O. ) Followed after, the solution was assorted within the Ziploc bag and it was poured into a clean beaker through a large ball of cheese fabrics that was folded twice for best filtration consequences. Afterwards, chilled 99 % ethyl alcohol was easy and carefully poured into the smaller beaker, non upseting the strawberry mixture ( ethanol droplets rolled down the sides of the beaker easy. ) Observations took topographic point after ethyl alcohol was poured, and the precipitate was collected with toothpicks. Consequences and observations were recorded. Consequences: Materials used BEFORE AFTER Strawberries Red, with seeds and green roots Smells fruity, and looked fresh Heated by microwave for about 15seconds Crushed by a liquidizer, Liquid, ruddy, strawberry slush Seeds and strawberry meat assorted together Buffer Clear liquid ; crystalline solution Nose-piercing odor ; smells a spot salty Mixed in with the strawberry solution Expressions like ruddy slush ; to the full assorted Ethyl alcohol Clear and crystalline liquid. Icy cold. Nose-piercing odor. 99 % intoxicant Alcohol creates another stage with the strawberry solution. One bed of clear liquid on top of strawberry juice. A seeable line of division between the two stages. Air bubbles were present Precipitate Clear, pinkish twine like DNA bunchs. Expressions like noodles. Have a smooth touch to it, and could be easy picked up with a toothpick. After adding the chilled ethyl alcohol to the filtered strawberry mixture, two stages became seeable ; ethanol stage and strawberry solution stage. There was a seeable spliting line between them. Within the in-between subdivision of the two stages, precipitate formed. String sections of clear and white gooey substance quickly rose to the surface of the ethyl alcohol while the remainder of the mixture remained the same. Elian Gonzalez Should Stay In The U.S. EssayThe ground why the ethyl alcohol was chilled and iced is because this would increase the output of DNA extracted. ( University of Queensland, 2011 ) Since Deoxyribonucleic acid strands are held together by H bonds, without the protection of atomic membranes and cell membranes, they will go really delicate in warm environments. ( Zumbo, 2012 ) Cold ethyl alcohol besides helps to decelerate down the activity of DNases, or limitation enzymes that protect cells from viruses by destructing any Deoxyribonucleic acid in the cytol. ( Zumbo, 2012 ) Therefore, chilled ethyl alcohol would protect the DNA strands from damaged and later taking to a greater output of extracted strawberry DNA. ( Buddies, 2013 ) Uses for Extracted Strawberry DNA Not merely does strawberry DNA provide pupils with acquisition and biological science cognition, it besides helps out the society in many ways. For illustration: Use the Deoxyribonucleic acid of a extremely disease-resistant strawberry’s cistrons for research intents ( Sweeney, 2012 ) Surveies go arounding the evolutionary history of strawberries could take topographic point ( Sweeney, 2012 ) Genetic research could besides take topographic point by analyzing the DNA strands of strawberries. ( Some chemicals in strawberries are known to decelerate down the growing of some tumours ) . ( Sweeney, 2012 ) Decision: This lab activity was a success, but there are some possible stairss to take for a higher output of extracted strawberry DNA. During the filtration procedure, pupils should hold filtered out the useless strawberry cell debris more carefully. This manner, they would hold gotten a clearer DNA solution, which would so ensue in a faster and more dynamic reaction between ethyl alcohol and the filtered solution. Merely like the anticipations in the beginning of this lab activity, the consequence was a big measure of clear, pinkish and slimed strands of Deoxyribonucleic acid that looked like spaghetti noodles. Despite of the simple process, the consequences were still highly obvious, even to the bare oculus. It was truly easy to see the white DNA strands within the reddish-pink strawberry juice solution. Overall, it was a successful and interesting experiment, and a nice sum of cognition was gained throughout the procedure. Bibliography Buddies. ( 2013, January 31 ) . Spongy Science: Extract Deoxyribonucleic acid from Smashed Strawberries.Scientific American. Retrieved March 29, 2014, from hypertext transfer protocol: //www.scientificamerican.com/article/squishy- science-extract-dna-from-smashed-strawberries/ Sweeney, D. ( 2012, October 20 ) . DNA Isolation from Strawberries.Department of Genome Sciences. Retrieved March 30, 2014, from hypertext transfer protocol: //www.gs.washington.edu/outreach/dhillon_dnaprocedure.pdf University of Queensland. ( 2011 ) The University of Queensland.Strawberry DNA extraction experiment. Retrieved March 30, 2014, from hypertext transfer protocol: //www.imb.uq.edu.au/strawberry-dna-extraction- experiment Wasserman, R. ( 2010, May 11 ) . Why Is Sodium Used in DNA Extraction? .eHow. Retrieved March 30, 2014, from hypertext transfer protocol: //www.ehow.com/about_6504902_sodium-used-dna-extraction_.html Zumbo. ( 2012 ) . Ethanol Precipitation.DEPARTMENT OF PHYSIOLOGY A ; BIOPHYSICS. Retrieved March 30, 2014, from hypertext transfer protocol: //physiology.med.cornell.edu/faculty/mason/lab/zumbo/files/ETHANOL_PRECIP itation.pdf